中华麻醉在线
您当前位置:主页 > 热点专题 > 中国发表SCI论文汇总 >

Cell Mol Neurobiol.:Atorvastatin Attenuates Ischemia/Reperfusion-Induced Hippocampal Neurons Injury Via Akt-nNOS-JNK Signaling Pathway.

2017-06-07 13:02  来源:未知  编辑:高国雷   点击:

Abstract

Ischemia-induced brain damage leads to apoptosis like delayed neuronal death in selectively vulnerable regions, which could further result in irreversible damages. Previous studies have demonstrated that neurons in the CA1 area of hippocampus are particularly sensitive to ischemic damage. Atorvastatin (ATV) has been reported to attenuate cognitive deficits after stroke, but precise mechanism for neuroprotection remains unknown. Therefore, the aims of this study were to investigate the neuroprotective mechanisms of ATV against ischemic brain injury induced by cerebral ischemia reperfusion. In this study, four-vessel occlusion model was established in rats with cerebral ischemia. Rats were divided into five groups: sham group, I/R group, I/R+ATV group, I/R+ATV+LY, and I/R+SP600125 group. Cresyl violet staining was carried out to examine the neuronal death of hippocampal CA1 region. Immunoblotting was used to detect the expression of the related proteins. Results showed that ATV significantly protected hippocampal CA1 pyramidal neurons against cerebral I/R. ATV could increase the phosphorylation of protein kinase B (Akt1) and nNOS, diminished the phosphorylation of JNK3 and c-Jun, and further inhibited the activation of caspase-3. Whereas, all of the aforementioned effects of ATV were reversed by LY294002 (an inhibitor of Akt1). Furthermore, pretreatment with SP600125 (an inhibitor of JNK) diminished the phosphorylation of JNK3 and c-Jun, and further inhibited the activation of caspase-3 after cerebral I/R. Taken together, our results implied that Akt-mediated phosphorylation of nNOS is involved in the neuroprotection of ATV against ischemic brain injury via suppressing JNK3 signaling pathway that provide a new experimental foundation for stroke therapy.

KEYWORDS:

Akt1; Cerebral ischemia; JNK3; LY294002; nNOS


相关推荐
网友评论
请自觉遵守互联网相关的政策法规,严禁发布色情、暴力、反动的言论。
评价:
用户名: 验证码: 点击我更换图片
 
Copyright 中华麻醉在线 All rights reserved
地址:北京市东城区东四西大街42号中华医学会南楼2单元222室麻醉学分会办公室 邮编:100710
联系人:白雪   电话:010-85158614   传真:010-85158753   京ICP备14001125
360网站安全检测平台